Journal: Oncology Letters

Article Title: miR-9-3p inhibits glioma cell proliferation and apoptosis by directly targeting FOXG1

doi: 10.3892/ol.2020.11725

Figure Lengend Snippet: Effects of miR-9-3p overexpression on the proliferation of U87 cells. U87 cells were transfected with miR-9-3p mimic and NC mimic (50 pmol/ml) for 24 h. (A and C) RT-qPCR was applied to quantify the expression level of miR-9-3p in U87 MG and TG-905 cells. (B and D) The cell viability of U87 cells was determined by a CCK-8 assay in U87 MG and TG-905 cells. ##P<0.01 vs. NC mimic group. miR-9-3p, microRNA-9-3p; RT-qPCR, reverse transcription-quantitative polymerase chain reaction; NC, negative control; control, without any treatment.

Article Snippet: Cell culture and transfection The human glioblastoma U87 MG (CL-0238) and TG-905 (CL-0309) cell lines were purchased from Procell Life Science & Technology Co., Ltd. (the origin of glioblastoma U87 MG cell is unknown and the cell line is preserved at the ATCC).

Techniques: Over Expression, Transfection, Quantitative RT-PCR, Expressing, CCK-8 Assay, Real-time Polymerase Chain Reaction, Negative Control

Journal: Oncology Letters

Article Title: miR-9-3p inhibits glioma cell proliferation and apoptosis by directly targeting FOXG1

doi: 10.3892/ol.2020.11725

Figure Lengend Snippet: Effects of miR-9-3p overexpression on the apoptosis of U87 cells. U87 cells were transfected with miR-9-3p mimic and NC mimic (50 pmol/ml) for 24 h. (A) Annexin-V/PI double-staining assay was applied to detect the cell apoptotic rate in U87 MG cells. (B) Annexin-V/PI double-staining assay was applied to detect the cell apoptotic rate in TG-905 cells. ##P<0.01 vs. NC mimic group. miR-9-3p, microRNA-9-3p; NC, negative control; PI, propidium iodide; control, without any treatment.

Article Snippet: Cell culture and transfection The human glioblastoma U87 MG (CL-0238) and TG-905 (CL-0309) cell lines were purchased from Procell Life Science & Technology Co., Ltd. (the origin of glioblastoma U87 MG cell is unknown and the cell line is preserved at the ATCC).

Techniques: Over Expression, Transfection, Double Staining, Negative Control

Journal: Oncology Letters

Article Title: miR-9-3p inhibits glioma cell proliferation and apoptosis by directly targeting FOXG1

doi: 10.3892/ol.2020.11725

Figure Lengend Snippet: FOXG1 is a direct target of miR-9-3p in glioma cells. (A) Bioinformatics analysis of the predicted interactions of miR-9-3p and its binding sites within the 3′-UTR of FOXG1. (B) Relative luciferase activities of FOXG1-wt, and FOXG1-mut were identified using a Dual-Luciferase Reporter assay kit following transfection of U87 MG with miR-9-3p mimic and NC mimic (50 pmol/ml) for 24 h. (C) The expression level of FOXG1 mRNA was examined by RT-qPCR following transfection of U87 MG with miR-9-3p mimic and NC mimic (50 pmol/ml) for 24 h. (D) The expression level of FOXG1 protein was examined by western blot assay following transfection of U87 MG with miR-9-3p mimic and NC mimic (50 pmol/ml) for 48 h. The relative intensity of FOXG1 protein is presented as a bar graph. (E) Relative luciferase activities of FOXG1-wt, and FOXG1-mut were identified using a Dual-Luciferase Reporter assay kit following transfection of TG-905 cells with miR-9-3p mimic and NC mimic (50 pmol/ml) for 24 h. (F) The expression level of FOXG1 mRNA was examined by RT-qPCR following transfection of TG-905 cells with miR-9-3p mimic and NC mimic (50 pmol/ml) for 24 h. (G) The expression level of FOXG1 protein was examined by western blot assay following transfection of TG-905 cells with miR-9-3p mimic and NC mimic (50 pmol/ml) for 48 h. The relative intensity of FOXG1 protein is presented as a bar graph. *P<0.05, **P<0.01 vs. NC mimic group. FOXG1, forkhead box G1; miR-9-3p, microRNA-9-3p; 3′-UTR, 3′-untranslated region; wt, wild-type; mut, mutant; NC, negative control.

Article Snippet: Cell culture and transfection The human glioblastoma U87 MG (CL-0238) and TG-905 (CL-0309) cell lines were purchased from Procell Life Science & Technology Co., Ltd. (the origin of glioblastoma U87 MG cell is unknown and the cell line is preserved at the ATCC).

Techniques: Binding Assay, Luciferase, Reporter Assay, Transfection, Expressing, Quantitative RT-PCR, Western Blot, Mutagenesis, Negative Control

Journal: Oncology Letters

Article Title: miR-9-3p inhibits glioma cell proliferation and apoptosis by directly targeting FOXG1

doi: 10.3892/ol.2020.11725

Figure Lengend Snippet: miR-9-3p regulates cell proliferation by inhibiting FOXG1. (A and C) Western blotting was used to detect the protein expression of FOXG1 following transfection of U87 MG and TG-905 cells with FOXG1 siRNA (40 pmol/ml) for 48 h. (B and D) Relative cell viability was determined by a Cell Counting Kit-8 assay following transfection of U87 MG and TG-905 cells with FOXG1 (40 pmol/ml) for 24 h. **P<0.01 vs. control group; ##P<0.01 vs. miR-9-3p mimic group; &&P<0.01 vs. FOXG1-siRNA group. FOXG1, forkhead box G1; miR-9-3p, microRNA-9-3p; siRNA, small interfering RNA; control, without any treatment.

Article Snippet: Cell culture and transfection The human glioblastoma U87 MG (CL-0238) and TG-905 (CL-0309) cell lines were purchased from Procell Life Science & Technology Co., Ltd. (the origin of glioblastoma U87 MG cell is unknown and the cell line is preserved at the ATCC).

Techniques: Western Blot, Expressing, Transfection, Cell Counting, Small Interfering RNA

Journal: Oncology Letters

Article Title: miR-9-3p inhibits glioma cell proliferation and apoptosis by directly targeting FOXG1

doi: 10.3892/ol.2020.11725

Figure Lengend Snippet: miR-9-3p regulates cell apoptosis by inhibiting FOXG1. (A) Annexin-V/PI double-staining assay was performed to detect the cell apoptotic rate of U87 MG cells following transfection with FOXG1 siRNA (40 pmol/ml) for 24 h. (B) Annexin-V/PI double-staining assay was applied to detect the cell apoptotic rate of TG-905 cells following transfection with FOXG1 siRNA (40 pmol/ml) for 24 h. **P<0.01 vs. control group; ##P<0.01 vs miR-9-3p mimic group; &&P<0.01 vs. FOXG1-siRNA group. miR-9-3p, microRNA-9-3p; FOXG1, forkhead box G1; PI, propidium iodide; siRNA, small interfering RNA; control, without any treatment.

Article Snippet: Cell culture and transfection The human glioblastoma U87 MG (CL-0238) and TG-905 (CL-0309) cell lines were purchased from Procell Life Science & Technology Co., Ltd. (the origin of glioblastoma U87 MG cell is unknown and the cell line is preserved at the ATCC).

Techniques: Double Staining, Transfection, Small Interfering RNA

Journal: Oncology Letters

Article Title: miR-9-3p inhibits glioma cell proliferation and apoptosis by directly targeting FOXG1

doi: 10.3892/ol.2020.11725

Figure Lengend Snippet: Effects of miR-9-3p overexpression on the proliferation of U87 cells. U87 cells were transfected with miR-9-3p mimic and NC mimic (50 pmol/ml) for 24 h. (A and C) RT-qPCR was applied to quantify the expression level of miR-9-3p in U87 MG and TG-905 cells. (B and D) The cell viability of U87 cells was determined by a CCK-8 assay in U87 MG and TG-905 cells. ##P<0.01 vs. NC mimic group. miR-9-3p, microRNA-9-3p; RT-qPCR, reverse transcription-quantitative polymerase chain reaction; NC, negative control; control, without any treatment.

Article Snippet: The human glioblastoma U87 MG (CL-0238) and TG-905 (CL-0309) cell lines were purchased from Procell Life Science & Technology Co., Ltd. (the origin of glioblastoma U87 MG cell is unknown and the cell line is preserved at the ATCC).

Techniques: Over Expression, Transfection, Quantitative RT-PCR, Expressing, CCK-8 Assay, Real-time Polymerase Chain Reaction, Negative Control

Journal: Oncology Letters

Article Title: miR-9-3p inhibits glioma cell proliferation and apoptosis by directly targeting FOXG1

doi: 10.3892/ol.2020.11725

Figure Lengend Snippet: Effects of miR-9-3p overexpression on the apoptosis of U87 cells. U87 cells were transfected with miR-9-3p mimic and NC mimic (50 pmol/ml) for 24 h. (A) Annexin-V/PI double-staining assay was applied to detect the cell apoptotic rate in U87 MG cells. (B) Annexin-V/PI double-staining assay was applied to detect the cell apoptotic rate in TG-905 cells. ##P<0.01 vs. NC mimic group. miR-9-3p, microRNA-9-3p; NC, negative control; PI, propidium iodide; control, without any treatment.

Article Snippet: The human glioblastoma U87 MG (CL-0238) and TG-905 (CL-0309) cell lines were purchased from Procell Life Science & Technology Co., Ltd. (the origin of glioblastoma U87 MG cell is unknown and the cell line is preserved at the ATCC).

Techniques: Over Expression, Transfection, Double Staining, Negative Control

Journal: Oncology Letters

Article Title: miR-9-3p inhibits glioma cell proliferation and apoptosis by directly targeting FOXG1

doi: 10.3892/ol.2020.11725

Figure Lengend Snippet: FOXG1 is a direct target of miR-9-3p in glioma cells. (A) Bioinformatics analysis of the predicted interactions of miR-9-3p and its binding sites within the 3′-UTR of FOXG1. (B) Relative luciferase activities of FOXG1-wt, and FOXG1-mut were identified using a Dual-Luciferase Reporter assay kit following transfection of U87 MG with miR-9-3p mimic and NC mimic (50 pmol/ml) for 24 h. (C) The expression level of FOXG1 mRNA was examined by RT-qPCR following transfection of U87 MG with miR-9-3p mimic and NC mimic (50 pmol/ml) for 24 h. (D) The expression level of FOXG1 protein was examined by western blot assay following transfection of U87 MG with miR-9-3p mimic and NC mimic (50 pmol/ml) for 48 h. The relative intensity of FOXG1 protein is presented as a bar graph. (E) Relative luciferase activities of FOXG1-wt, and FOXG1-mut were identified using a Dual-Luciferase Reporter assay kit following transfection of TG-905 cells with miR-9-3p mimic and NC mimic (50 pmol/ml) for 24 h. (F) The expression level of FOXG1 mRNA was examined by RT-qPCR following transfection of TG-905 cells with miR-9-3p mimic and NC mimic (50 pmol/ml) for 24 h. (G) The expression level of FOXG1 protein was examined by western blot assay following transfection of TG-905 cells with miR-9-3p mimic and NC mimic (50 pmol/ml) for 48 h. The relative intensity of FOXG1 protein is presented as a bar graph. *P<0.05, **P<0.01 vs. NC mimic group. FOXG1, forkhead box G1; miR-9-3p, microRNA-9-3p; 3′-UTR, 3′-untranslated region; wt, wild-type; mut, mutant; NC, negative control.

Article Snippet: The human glioblastoma U87 MG (CL-0238) and TG-905 (CL-0309) cell lines were purchased from Procell Life Science & Technology Co., Ltd. (the origin of glioblastoma U87 MG cell is unknown and the cell line is preserved at the ATCC).

Techniques: Binding Assay, Luciferase, Reporter Assay, Transfection, Expressing, Quantitative RT-PCR, Western Blot, Mutagenesis, Negative Control

Journal: Oncology Letters

Article Title: miR-9-3p inhibits glioma cell proliferation and apoptosis by directly targeting FOXG1

doi: 10.3892/ol.2020.11725

Figure Lengend Snippet: miR-9-3p regulates cell proliferation by inhibiting FOXG1. (A and C) Western blotting was used to detect the protein expression of FOXG1 following transfection of U87 MG and TG-905 cells with FOXG1 siRNA (40 pmol/ml) for 48 h. (B and D) Relative cell viability was determined by a Cell Counting Kit-8 assay following transfection of U87 MG and TG-905 cells with FOXG1 (40 pmol/ml) for 24 h. **P<0.01 vs. control group; ##P<0.01 vs. miR-9-3p mimic group; &&P<0.01 vs. FOXG1-siRNA group. FOXG1, forkhead box G1; miR-9-3p, microRNA-9-3p; siRNA, small interfering RNA; control, without any treatment.

Article Snippet: The human glioblastoma U87 MG (CL-0238) and TG-905 (CL-0309) cell lines were purchased from Procell Life Science & Technology Co., Ltd. (the origin of glioblastoma U87 MG cell is unknown and the cell line is preserved at the ATCC).

Techniques: Western Blot, Expressing, Transfection, Cell Counting, Small Interfering RNA

Journal: Oncology Letters

Article Title: miR-9-3p inhibits glioma cell proliferation and apoptosis by directly targeting FOXG1

doi: 10.3892/ol.2020.11725

Figure Lengend Snippet: miR-9-3p regulates cell apoptosis by inhibiting FOXG1. (A) Annexin-V/PI double-staining assay was performed to detect the cell apoptotic rate of U87 MG cells following transfection with FOXG1 siRNA (40 pmol/ml) for 24 h. (B) Annexin-V/PI double-staining assay was applied to detect the cell apoptotic rate of TG-905 cells following transfection with FOXG1 siRNA (40 pmol/ml) for 24 h. **P<0.01 vs. control group; ##P<0.01 vs miR-9-3p mimic group; &&P<0.01 vs. FOXG1-siRNA group. miR-9-3p, microRNA-9-3p; FOXG1, forkhead box G1; PI, propidium iodide; siRNA, small interfering RNA; control, without any treatment.

Article Snippet: The human glioblastoma U87 MG (CL-0238) and TG-905 (CL-0309) cell lines were purchased from Procell Life Science & Technology Co., Ltd. (the origin of glioblastoma U87 MG cell is unknown and the cell line is preserved at the ATCC).

Techniques: Double Staining, Transfection, Small Interfering RNA